Plant Cell Culture: Essential Methods

The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf.

• Highlights the best and most up-to-date techniques for working on plant cell culture
• Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches
• Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture

An essential laboratory manual for students and early-career researchers.Content:
Chapter 1 Plant Micropropagation (pages 1–23): Ivan Iliev, Alena Gajdosova, Gabriela Libiakova and Shri Mohan Jain
Chapter 2 Thin Cell Layers: The Technique (pages 25–37): Jaime A. Teixeira da Silva and Michio Tanaka
Chapter 3 Plant Regeneration – Somatic Embryogenesis (pages 39–59): Kim E. Nolan and Ray J. Rose
Chapter 4 Haploid Plants (pages 61–78): Sant S. Bhojwani and Prem K. Dantu
Chapter 5 Embryo Rescue (pages 79–95): Traud Winkelmann, Antje Doil, Sandra Reinhardt and Aloma Ewald
Chapter 6 In vitro Flowering and Seed Set: Acceleration of Generation Cycles (pages 97–110): Sergio J. Ochatt and Rajbir S. Sangwan
Chapter 7 Induced Mutagenesis in Plants Using Physical and Chemical Agents (pages 111–130): Chikelu Mba, Rownak Afza, Souleymane Bado and Shri Mohan Jain
Chapter 8 Cryopreservation of Plant Germplasm (pages 131–151): E. R. Joachim Keller and Angelika Senula
Chapter 9 Plant Protoplasts: Isolation, Culture and Plant Regeneration (pages 153–173): Michael R. Davey, Paul Anthony, Deval Patel and J. Brian Power
Chapter 10 Protoplast Fusion Technology – Somatic Hybridization and Cybridization (pages 175–198): Jude W. Grosser, Milica calovic and Eliezer S. Louzada
Chapter 11 Genetic Transformation – Agrobacterium (pages 199–215): Ian S. Curtis
Chapter 12 Genetic Transformation – Biolistics (pages 217–239): Fredy Altpeter and Sukhpreet Sandhu
Chapter 13 Plastid Transformation (pages 241–260): Bridget V. Hogg, Cilia L. C. Lelivelt, Aisling Dunne, Kim?Hong Nguyen and Jacqueline M. Nugent
Chapter 14 Molecular Characterization of Genetically Manipulated Plants (pages 261–279): Cristiano Lacorte, Giovanni Vianna, Francisco J. L. Aragao and Elibio L. Rech
Chapter 15 Bioreactors (pages 281–296): Spiridon Kintzios
Chapter 16 Secondary Products (pages 297–315): Kexuan Tang, Lei Zhang, Junfeng Chen, Ying Xiao, Wansheng Chen and Xiaofen Sun
Chapter 17 Plant Cell Culture – Present and Future (pages 317–331): Jim M. Dunwell